Featured
- Get link
- X
- Other Apps
Escherichia Coli Acid Fast Stain
Escherichia Coli Acid Fast Stain. (1) bacteria are decolorizes by acid alcohol and do not retain the initial stain, carbolfuchsin, (2) so it can pick up the counterstain, methylene blue. Then the slide is kept undisturbed for 5 minutes and allows it to cool.

Make smear on a clean glass slide. Cover the smear with a strong carbol fuchsin solution. Escherichia coli is an example of acid fast bacteria.
Rinse The Slide With Distilled Water And Wash The Stain.
(1) bacteria are decolorizes by acid alcohol and do not retain the initial stain, carbolfuchsin, (2) so it can pick up the counterstain, methylene blue. Afb cell wall contains wax & pg carbolfuchsin stain & heat mycobacterium tuberculosis & m. Rinsing slide after application of crystal violet counterstain;
27 Lab 3 Results I.
(wait for nearly five minutes) Coli and tsa slant of mycobacterium. Acid fast and non acid fast bacteria are two types of bacteria, which can be differentiated based on the presence of mycolic acid in the cell wall of the bacteria.
That Is Why It Is Stained Blue Instead Of Red.
Bacillus subtilis candida albicans corynebacterium diptheriae enterobacter aerogenes escherichia coli enterococcus faecalis haemophilus. Cover the smear with a strong carbol fuchsin solution. Sterilize an inoculating loop and allow it to cool.
Washing Is Followed By Acid Fast Decolourizer (3% V/V Acid Alcohol) Treatment For About 2 Minutes.
What is the purpose of heating the slide during the staining process? Specific stain method for mycobacterium tuberculosis, also known as. Links to the gram stain and/or acid fast stain of your unknown.
Cultural Characteristics Of Escherichia Coli:
Then the slide is kept undisturbed for 5 minutes and allows it to cool. Make smear on a clean glass slide. Obtain a clean glass slide.
Popular Posts
Duraseal Weathered Oak Stain On Red Oak
- Get link
- X
- Other Apps
How To Fix Bleach Stain On Black Shirt
- Get link
- X
- Other Apps
Comments
Post a Comment